PCR-amplified Immunoassay (Immuno-PCR): Principle of the Method, Variants of Execution, Possibilities and Prospects of Use for the Detection of Pathogenic Biological Agents

Enzyme immunoassay and polymerase chain reaction have become the «gold standard» for detection of biological pathogens. The method amplified – immuno-PCR allows to combine both methods into a single platform preserve their advantages achieve high sensitivity analysis. purpose this work is consider possibilities prospects using PCR-amplified pathogenic agents. Immuno-PCR makes it possible detect various non-nucleic antigenic determinants in PCR by amplifying DNA tag conjugated with specific antibody. registration results also real time as real-time test systems. main methodological issues technology are: choice carrier biomolecule complexes, conjugation antibodies reporter nucleic acid, optimization signal accounting results, development reducing background indicators. We necessary carry out research on creation diagnostic kits based immuno-PCR. With regard task detecting small trace amounts antigens agents, most likely «niche» will be toxins microbial non-microbial origin, minimum clinically significant dose which less than corresponding immunochemical Taking account method, future develop such systems hapten analytes, example, some toxicants non-biological origin